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M9480502.TXT
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1994-08-20
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Document 0502
DOCN M9480502
TI Characterization of human immunodeficiency virus type 1 dimeric RNA from
wild-type and protease-defective virions.
DT 9410
AU Fu W; Gorelick RJ; Rein A; ABL-Basic Research Program, NCI-Frederick
Cancer Research and; Development Center, Maryland 21702-1201.
SO J Virol. 1994 Aug;68(8):5013-8. Unique Identifier : AIDSLINE
MED/94309166
AB We have characterized the dimeric genomic RNA in particles of both
wild-type and protease (PR)-deficient human immunodeficiency virus type
1 (HIV-1). We found that the dimeric RNA isolated from PR- mutant
virions has a lower mobility in nondenaturing gel electrophoresis than
that from wild-type virions. It also dissociates into monomers at a
lower temperature than the wild-type dimer. Thus, the dimer in PR-
particles is in a conformation different from that in wild-type
particles. These results are quite similar to recent findings on Moloney
murine leukemia virus and suggest that a postassembly, PR-dependent
maturation event is a common feature in genomic RNAs of retroviruses. We
also measured the thermal stability of the wild-type and PR- dimeric
RNAs under different ionic conditions. Both forms of the dimer were
stabilized by increasing Na+ concentrations. However, the melting
temperatures of the two forms were not significantly affected by the
identity of the monovalent cation present in the incubation buffer. This
observation is in contrast with recent reports on dimers formed in vitro
from short segments of HIV-1 sequence: the latter dimers are
specifically stabilized by K+ ions. K+ stabilization of dimers formed in
vitro has been taken as evidence for the presence of guanine quartet
structures. The results suggest that guanine quartets are not involved
in the structure linking full-length, authentic genomic RNA of HIV-1
into a dimeric structure.
DE Biopolymers Hela Cells Human HIV Protease/*METABOLISM
HIV-1/ENZYMOLOGY/*GENETICS Nucleic Acid Conformation RNA,
Viral/*CHEMISTRY Sodium Chloride Support, U.S. Gov't, P.H.S.
Temperature Virion/ENZYMOLOGY/GENETICS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).